|Chromogen of acidic stain is negatively charged. It is also referred to as anionic stain.
|Chromogen or colored part of basic stain is positively charged. It is also referred to as cationic stain.
|It is a complex of dye acid with dye base.
|Used to stain the positively charged component of microbial cell.
|Used to stain negatively charged component of microbial cell.
|It stains both positive and negative charged components of microbial cell.
|Example: Eosin, Nigrosin, India ink, Acidic fuchsin, Congo red etc.
|Example: Methylene blue, Safranin, Malachite green, Basic fuchsin, Crystal violet etc.
|Example: Giemsa stain, Leishmanstain etc.