Acid Stain Vs Basic Stain Vs Neutral Stain: What Is The Difference?

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Acid Stain Basic Stain Neutral Stain
Chromogen of acidic stain is negatively charged. It is also referred to as anionic stain. Chromogen or colored part of basic stain is positively charged. It is also referred to as cationic stain. It is a complex of dye acid with dye base.
Used to stain the positively charged component of microbial cell. Used to stain negatively charged component of microbial cell. It stains both positive and negative charged components of microbial cell.
Example: Eosin, Nigrosin, India ink, Acidic fuchsin, Congo red etc. Example: Methylene blue, Safranin, Malachite green, Basic fuchsin, Crystal violet etc. Example: Giemsa stain, Leishmanstain etc.

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